Review on In vitro formulation of Microsphere containing Andrographis Pinaculata

Ms.Saloni Saw
Faculty of Pharmacy Kalinga University,
Naya Raipur Chhattisgarh
Email id- salonisaw@kalingauniversity.ac.in
To increase the bioavailability and maintain the release of its active ingredient, andrographolide, which has a restricted oral bioavailability because of its weak water solubility, microspheres containing extract from Andrographis paniculata have been developed. Microspheres can aid by enabling regulated release, protecting the active ingredient, and improving therapeutic efficacy. In conclusion, meticulous extraction, polymer selection, and systematic processing are necessary to formulate microspheres for Andrographis paniculata to provide a controlled release system. By improving the therapeutic advantages of andrographolide, this delivery system seeks to make its use in medical therapies more efficient.
Introduction
To increase bioavailability, stability, and the capacity to deliver drugs to particular sites at a predefined pace, multi-particulate drug delivery systems called microspheres are created to provide extended or controlled drug delivery. They are constructed from natural, semi-synthetic, synthetic polymers, polymeric waxy compounds, and other protective components. Microspheres are made of synthesized or proteinous polymers and have particle sizes ranging from 1 to 1000 μm. They are distinguished by their free-flowing nature [1].
Material Method
The solvent evaporation technique was used to create various ratios of Andrographis Pinaculata microspheres utilizing the polymers Eudragit RSPO, Ethyl cellulose N22, and Ethyl cellulose 100cps. To create microspheres, Andrographis Pinaculata and firstly, polymers were dissolved in acetone in the following ratios: 1:1, 1:2, and 1:0.5. The resulting solution was then gradually dispersed in heavy liquid paraffin while being continuously stirred at two different temperatures, namely room temperature and 40–45°C.Using a mechanical stirrer, the mixture was vortexed for 15 to 30 minutes at a high RPM. After filtering off the paraffin, the resulting microspheres were cleaned three times with petroleum ether, once more with water, dried, and gathered [2].
Evaluation of Microsphere
Analysis of surface structure
Analysis of particle size
Evaluation of medication loading and encapsulation effectiveness
FTIR spectroscopy, or Fourier Transform Infrared
Drug release in vitro
Conclusion
The process of making microspheres with Andrographis paniculata includes developing controlled-release delivery methods that improve the active ingredients’ bioavailability and prolonged release, especially andrographolide, the primary bioactive ingredient. A broad approach to the in vitro production of such microspheres is provided here, with an emphasis on encapsulating A. paniculata extract utilizing polymer-based methods.
Reference
Gurung BD, Kakar S. An overview on microspheres. Int J Health Clin Res. 2020;3(1):11-24.
Reddy BV, Krishnaveni K. Formulation and evaluation of efavirenz microspheres. Der Pharmacia letters. 2015;7(6):1-9.
Uyen NT, Hamid ZA, Ahmad NB. Synthesis and characterization of curcumin loaded alginate microspheres for drug delivery. Journal of Drug Delivery Science and Technology. 2020 Aug 1;58:101796.